Photographs of Aequorea individuals and purified fluorescent proteins.

(A) White-light (i) and fluorescence (400-nm LED illumination) (ii) photographs of A. victoria and white-light photographs of A. cf. australis (iii, iv). The blue coloration of A. cf. australis is shown in the higher magnification image of one of its tentacle bulbs (iv). (B) Purified recombinant proteins from Aequorea species, shown under white light and 480-nm LED without emission filters. Protein concentrations were adjusted to display similar optical density as judged by eye and were between 0.5 and 2 mg/ml for all samples.